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微信小章| 产品名称： | pJCZ3.1 RW-164 |
|---|---|
| 商品货号： | TS139099 |
| Designations： | pJCZ3.1 RW-164 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | RFLPs observed under normal stringency (0.1X SSC). |
| Vector： | Construct size (kb): 8.600000381469727 |
| Insert： | DNA: genomic Insert lengths(kb): 6.0 Gene product: DNA Segment, single copy D19S20 |
| Insert Size (kb)： | 6.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--7.9, 0.71; EcoRI--8.6; HindIII--8.6; BamHI--4.3 doublet; HincII--7.6, 0.95; EcoRI/HindIII--6.0, 2.7; consistent with deposited DNA. The structure of this plasmid is not consistent with the published description of pJCZ3.1. The hybridization pattern of this probe with human DNA was not checked by the ATCC. Subcloned from cosmid JCZ3 isolated by zeta globin oligonucleotide. RFLPs observed under normal stringency (0.1X SSC). |
| References： | Odelberg SJ, et al. Characterization of eight VNTR loci by agarose gel electrophoresis. Genomics 5: 915-924, 1989. PubMed: 2574152 Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pJCZ3.1 on chromosome 19 D19S20. Nucleic Acids Res. 16: 1229, 1988. PubMed: 2894014 Peter J OConnell, personal communication |