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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS139154 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | DG101 CMCC 1963 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Preceptrol®： | no |
| Genotype： | F- thi- endA supE44 hsdR17 lacIq delta(lacZ)M15 pro+ tetS lambda- |
| Type Strain： | no |
| Comments： | A proC+ derivative of DG99 (a derivative of MM294) and isolated by D. Gelfand. A host strain providing efficient transformation and alkaline SDS screening of plasmid recombinants. It has a lac alpha acceptor for blue/white colony discrimination. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | JC Hunter-Cevera |
| References： | Lawyer FC, et al. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J. Biol. Chem. 264: 6427-6437, 1989. PubMed: 2649500 Jennie C Hunter-Cevera, personal communication |