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| 产品名称： | YEp24 pRB5 |
|---|---|
| 商品货号： | TS139205 |
| Designations： | YEp24 pRB5 |
| Depositors： | D Botstein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.7690000534057620 Vector: YEp24 (plasmid) Promoters: Promoter none Construction: pBR322, Saccharomyces cerevisiae, 2 micron ori Marker(s):URA3,ampR,tetR Construct size (kb): 7.769000053405762 Features: marker(s): URA3 marker(s): ampR marker(s): tetR promoter: none replicon: 2 micron replicon: pMB1 terminator: none enhancer: none |
| Applications： | YE-type (episomal) shuttle vector shuttle vector vector for constructing genomic libraries |
| Comments： | YE-type episomal shuttle vector. Plasmid was constructed by inserting a 2.2 kb EcoRI 2 micron B fragment and a 1.1 kb HindIII URA3 fragment into the EcoRI and HindIII sites of pBR322. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030 Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863 |
| Related Products： | component of:ATCC 87635 |
| Shipped： | frozen |
| Shipping Information： | Shipped: xa0Frozen glycerol stock of E. coli containing the plasmid. |