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| 产品名称： | lambdaS6 pcD-GALT |
|---|---|
| 商品货号： | TS139258 |
| Designations： | lambdaS6 pcD-GALT |
| GenBank Number： | M12955 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 47.0 |
| Insert： | DNA: genomic Insert lengths(kb): 18.0 Tissue: T-lymphoma SUP-T1 cell line Gene product: T cell receptor, gamma, variable region V3 TCRGJ2 Alleles: J2, G1, G2, J1 |
| Insert Size (kb)： | 18.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--20.0, 17.0, 9.2, 0.96; SacI--20.0, 9.2, 4.9 (doublet), 4.4 (doublet); XhoI--20.0, 18.0, 9.2; SmaI--20.0, 14.0, 7.2, 5.8; PvuI--23.0, 12.0, 8.2. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRI--2.9, 4.6, 11.2, 13.6, 14.5. Contains a productive rearrangement of Vgamma4 and Jgamma2. The insert begins about 4.3 kb upstream of Vgamma2 and extends 3.8 kb beyond TCRGJ2. The insert extends 3.3 kb more in the 5 and is 1.9 kb shorter in the 3 end than lambdaS13. Additional GenBank/EMBL accession numbers: M13429, M13430. |
| References： | LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743 Marie-Paule Lefranc, personal communication |