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微信小章| 产品名称： | phPAH247 PAH247 |
|---|---|
| 商品货号： | TS139261 |
| Designations： | phPAH247 PAH247 |
| GenBank Number： | K03020 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.800000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.400000095367432 Tissue: liver Gene product: phenylalanine hydroxylase PAH Alleles: A1, A2, E1, G1, G2, K1, K2, C1, C2, C3, D1, D2, E2, F2, J1, J2, F1, A2 |
| Insert Size (kb)： | 2.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.4; HindIII--5.9, 0.65; PvuII--3.6, 3.2; BamHI--5.1, 1.7; EcoRV--6.8. Restriction sites (with nucleotide positions relative to the published sequence) include: BamHI--1038; BglII--511; HindIII--1311, 1809; PstI--928; PvuII--902, 1610; TaqI--749; XbaI--423; XhoI--748. The following restriction enzymes do not cut the insert: AatII, AhaII, ApaI, BclI, BglI, BstEII, BstXI, BvuI, ClaI, EcoRI, EcoRV, HaeII, HgaI, KpnI, MluI, MstI, NarI, NruI, PvuI, SacI, SacII, SalI, SfiI, SphI, SstI, SstII, StuI, TthI, XmaIII, and XmnI. This insert contains a complete coding sequence with translation initiating at nucleotide 223 and ending at nucleotide 1579. The allele frequencies for the MspI, EcoRI, BglII, PvuII, EcoRV, HindIII, and XmnI are markedly different between Caucasian and Japanese populations. |
| References： | Kwok SC, et al. Nucleotide sequence of a full-length complementary cDNA clone and amino acid sequence of human phenylalanine hydroxylase. Biochemistry 24: 556-561, 1985. PubMed: 2986678 Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597 Detera-Wadleigh SD, et al. A TthIII1 polymorphism on the 5 flanking region of the glucocorticoid receptor gene (GRL). Nucleic Acids Res. 19: 1960, 1991. PubMed: 1674374 Savio L Woo, personal communication |