产品名称: | Human herpesvirus 1 |
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商品货号: | TS139282 |
Classification: | Herpesviridae, Simplexvirus |
Agent: | Human herpesvirus 1 |
Strain: | MP mutant strain of herpes simplex virus type 1 |
Common Name: | Herpes simplex virus 1 |
Applications: | Sexually Transmitted Disease Research |
Biosafety Level: | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format: | frozen |
Storage Conditions: | -70°C or colder |
Comments: | DNA of strain MP is about 95 X 10(6) daltons in molecular weight--4 million less than wild type. |
Effect on Host: | CPE (syncytial areas forming large plaques, monolayer sloughing) |
Recommended Host: | A549 (ATCC® CCL-185™) |
Growth Conditions: | Temperature: 37°C Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80%-90% confluent (not 100% confluent). Incubation: 1-3 days |
Name of Depositor: | B. Roizman |
Source: | ATCC® VR-1383™ was derived by passage of ATCC® VR-735™ in presence of Mycoplasma Removal Agent (ICN) to remove contaminating mycoplasma. Human tissue/FL cell culture mutant of type 1 strain selected on basis of its ability to cause cell fusion. |
References: | Hoggan MD, Roizman B. The isolation and properties of a variant of Herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody. Am. J. Hyg. 70: 208-219, 1959. PubMed: 14402487 Roizman B, et al. Herpes simplex viruses I and II: a biochemical definition. Perspect. Virol. 8: 129-169, 1973. |