产品名称: | c9214 9214 |
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商品货号: | TS139333 |
Designations: | c9214 9214 |
Species: | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
Depositors: | MV Olson, L Riles |
Vector: | Construct size (kb): 0.0 |
Insert: | DNA: genomic Gene product: 3-isopropylmalate dehydrogenase(beta-isopropylmalate dehydrogenase) LEU2 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Related Products: | component of:ATCC 77284 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Comments: | Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.399, 2.265, 4.747, 1.202, 1.816, 4.232, 2.260, 1.414, 3.064, 3.478, 3.682. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 1.2, 1.4, 1.9, 2.3, 3.2, 3.7, 3.8, 4.3, 5.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70303, 70648, 70649. |
Classification: | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
References: | Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 |