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| 产品名称： | HGR4.5 HUMGRA1 |
|---|---|
| 商品货号： | TS139391 |
| Designations： | HGR4.5 HUMGRA1 |
| GenBank Definition： | Human glucocorticoid receptor gene, exon 1 |
| GenBank Number： | M73050 |
| Species： | Homo sapiens, human |
| Depositors： | MV Govindan |
| Vector： | Construct size (kb): 8.90000057220459 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: EcoRI Vector end: EcoRI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GRL Genomic copy number: unique Gene name: glucocorticoid receptor Contains complete coding sequence?: U Chromosome: 5; Localization: 5 q31-q32 Tissue: leukocyte Type of DNA: genomic Insert end: EcoRI Insert end: EcoRI Insert size (kb): 4.5 Cross references: DNA Seq. Acc.: M73050 Insert lengths(kb): 4.5 Tissue: leukocyte Gene product: glucocorticoid receptor GRL |
| Insert Size (kb)： | 4.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.8, 4.5; HindIII--9.3; PstI--6.2, 3.0; SacI--8.6, 0.52; XbaI--9.3. The insert contains the following restriction sites (approximate kb from the 5 end: SmaI--1.8, 3.4; SacI--2.1, 2.6; PstI--2.4; NcoI--2.8, XbaI--4.1. Contains the promoter region, exon 1 and part of intron 1. Subcloned from lambdaGHGR-5. TS139391/9 and ATCC 80010/11 are contiguous in the genome. |
| References： | Govindan MV, et al. Human glucocorticoid receptor gene promoter-homologous down regulation. J. Steroid Biochem. Mol. Biol. 40: 317-323, 1991. PubMed: 1958537 Leclerc S, et al. Purification of a human glucocorticoid receptor gene promoter-binding protein. J. Biol. Chem. 266: 8711-8719, 1991. PubMed: 2026589 |