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Dermal Cell Basal Medium

货号 TS139396
中文名称 null
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产品名称: Dermal Cell Basal Medium
商品货号: TS139396
Product Format: 485 mL
Storage Conditions: 2 to 8°C, protect from light
Shipping Information: room temperature
Applications: Dermal Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCCxa0Primary Cell Solutions™xa0system.

This system is designed to support cells (e.g., keratinocytes, melanocytes) derived from normal human epidermis. Dermal Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.xa0

To support the proliferation and plating efficiency of keratinocytes (e.g., Primary Epidermal Keratinocytes, Normal, Human Neonatal Foreskin, ATCCxa0PCS-200-010), Dermal Cell Basal Medium must be supplemented with the Keratinocyte Growth Kit (ATCCxa0PCS-200-040). The complete serum-free (although not animal-free) media is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation.

To support the proliferation and plating efficiency of melanocytes (e.g., Primary Epidermal Melanocytes, Normal, Human Neonatal, ATCCxa0PCS-200-012 or Primary Epidermal Melanocytes, Normal, Human Adult, ATCCxa0PCS-200-013), Dermal Cell Basal Medium must be supplemented with either the Melanocyte Growth Kit (ATCCxa0PCS-200-041) or the Adult Melanocyte Growth Kit (ATCCxa0PCS-200-042).

Using Dermal Cell Basal Medium supplemented with the appropriate growth kit, the growth of keratinocytes and melanocytes are supported without the use of feeder layers, extracellular matrix proteins or other substrates.

Optional media supplements:

  1. Gentamicin-Amphotericin B Solution (ATCC PCS-999-025)
  2. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
  3. Phenol Red (ATCC PCS-999-001)

xa0

Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications:

Cell testing: Rate of proliferation and morphology.

pH: 7.5 ± 0.2
Osmolality: 315 ± 10 mOsm
Endotoxin: < 0.5 EU/mL
Sterility Testing:
Negative for bacteria, fungi and yeast.
Functional Testing: xxx