Construct size (kb): 5.400000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens DNA Segment, single copy, expressed
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: T08901; EST: EST06793; Primer: M13-21; THC
id: THC83492
DNA Seq. Acc.: T08902; EST: EST06794; Primer: M13 Reverse
DNA Seq. Acc.: X61451
Nucleotides 1-60, 60-371 of the insert correspond to
nucleotides 281-340, 339-650 of X61451 with 94% identity.

Insert lengths(kb): 2.099999904632568

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: DNA Segment, single copy, expressed F41*

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--5.6; HindIII/NotI--3.3, 2.1.

Based on the alignment of EST06794 with GenBank accession X61451 (mouse brain F41 transcript, 1040 nt, CDS 82-855), this clone appears to contain part of the coding region and 3 untranslated sequence.