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| 产品名称： | pCU--1 |
|---|---|
| 商品货号： | TS139430 |
| Designations： | pCU--1 |
| Species： | Candida tropicalis (Castellani) Berkhout |
| Depositors： | Henkel Research Corp., S Picataggio, Henkel Research Corp. |
| Vector： | Construct size (kb): 16.5 |
| Insert： | DNA: genomic Insert lengths(kb): 5.800000190734863 Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5-phosphate decarboxylase, orotate phosphoribosyltransferase 1) URA3 |
| Insert Size (kb)： | 5.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.8, 4.4 (doublet), 2.3, 0.4; EcoRI/HindIII--4.4 (doublet), 2.3, 1.6, 1.4 (doublet), 1.25, 0.75, 0.4; BamHI--uncut; BglI--11.0, 5.0, 2.4; EcoRV--7.2, 3.9, 3.4, 2.2, 1.0, 0.3; PstI--9.6, 4.3, 2.6, 1.0 (doublet). This plasmid complements the pyrF mutation in E. coli. A 6.2 kb EcoRI fragment contains 377 bp of vector sequence and all of the insert except for 50 bp. The gene is in a 2.8 kb EcoRI/SacI fragment. |
| References： | Picataggio S, et al. Site-specific modification of the Candida tropicalis genome. US Patent 5,254,466 dated Oct 19 1993 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |