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| 产品名称： | Besnoitia tarandi |
|---|---|
| 商品货号： | TS139432 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | clinical specimen - animal Isolation date: November, 2003 |
| Product Format： | frozen |
| Storage Conditions： | liquid nitrogen vapor phase |
| Type Strain： | no |
| Comments： | The parasite infects reindeer, caribou, musk ox and interferon gene knock out mice. The strain can be passaged in knockout mice by parenteral inoculation of tissue (liver, lung) extract from dead mice. |
| Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
|
| Growth Conditions： | Temperature: 37.0°C Duration: grown on monolayers of CV-1 cells (see ATCC CCL-70) in the presence of 50 micrograms/ml gentamicin Protocol: supernatant fluid from infected culture added to fresh monolayer of CV-1 cells Interval: 7 to 10 days |
| Subcultivation： | Protocol: supernatant fluid from infected culture added to fresh monolayer of CV-1 cells Interval: 7 to 10 days |
| Cryopreservation： | Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor： | JP Dubey |
| Year of Origin： | November, 2003 |
| References： | isolated from a naturally-infected reindeer (Rangifer tarandus) from Finland |