微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pBSL15 |
|---|---|
| 商品货号： | TS139450 |
| Designations： | pBSL15 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.9000000953674320 Vector: pBSL15 (plasmid) Construction: pUC18, pK18 Marker(s):ampR,kanR Construct size (kb): 3.900000095367432 Features: marker(s): ampR marker(s): kanR replicon: pMB1 MCS: HindIII...EcoRI MCS: NheI...HindIII |
| Applications： | contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance vector permitting positive selection for inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 1.2; BamHI--2.75, 1.3; HindIII--2.7, 1.4. The nptII gene was modified by site-directed mutagenesis to remove PstI and SphI sites. An inverted repeat of the 271 bp NdeI/EcoRI fragment of pUC18 surrounds the kanR cassette. If this vector is ligated without a stuffer fragment, this inverted repeat prevents replication, thus providing positive selection for plasmids with inserts. Plasmid with an easily purified kanR cassette (flanked by inverted repeats of the pUC18 multiple cloning sequence) for construction. pBSL15 and pBSL86 differ in the orientation of the repeats. |
| Media： | LB plus ampicillin (50 mcg/ml) plus kanamycin (50 mcg/ml)
|
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF. Three kanamycin resistance gene cassettes with different polylinkers. BioTechniques 18: 52-55, 1995. PubMed: 7702853 |
| Shipped： | freeze-dried |