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| 产品名称： | pLV57 |
|---|---|
| 商品货号： | TS139530 |
| Designations： | pLV57 |
| Depositors： | D OConnor |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.0999999046325680 Vector: pLV57 (plasmid) Construction: pBR325, NTP14m07(ts) Marker(s):ampR,cmlR,EcoRIm(ts)r+ Construct size (kb): 6.099999904632568 Features: insert detection: R+Mts marker(s): ampR, cmlR, EcoRIm(ts)r+ replicon: pMB1 |
| Applications： | vector permitting positive selection for inserts |
| Comments： | Restriction digests of the clone give the following sizes (kb): HincII--6.2; HindIII/BamHI--6.2; BamHI--uncut. Positive selection cloning vector employing EcoRI restriction-modification system. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | OConnor CD, Humphreys GO. Expression of the EcoRI restriction-modification system and the construction of positive-selection cloning vectors. Gene 20: 219-229, 1982. PubMed: 6299889 |
| Shipped： | freeze-dried |