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| 产品名称： | pW60 |
|---|---|
| 商品货号： | TS139536 |
| Designations： | pW60 |
| Depositors： | T Lufkin |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.6700000762939450 DESCRIPTION OF VECTOR: Intact vector size: 6.670 Type of vector: plasmid Cloning sites: NotI, XbaI Polylinker sites: Construction: pBluescript KS+ Host range: mammalian cells; Escherichia coli Features (with orientation and position when available): other: internal ribosome entry sequence (IRES), -> reporter group: lacZ, -> replicon: pMB1 marker(s): ampR Vector: pW60 (plasmid) Construction: pBluescript KS+ Marker(s):ampR Construct size (kb): 6.670000076293945 Features: marker(s): ampR other: internal ribosome entry sequence (IRES) replicon: pMB1 reporter group: lacZ |
| Applications： | reporter construct beta-galactosidase beta-D-galactosidase |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.6, 3.1; HindIII--6.4, 0.3. Plasmid expressing beta-galactosidase. Foreign genes can be cloned upstream of the IRES/reporter gene, allowing production of a dicistronic messenger RNA in mammalian cells. IRES/reporter insert can be released by XhoI. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Li X, et al. Dicistronic lacZ and alkaline phosphatase reporter constructs permit simultaneous histological analysis of expression from multiple transgenes. BioTechniques 23: 874-882, 1997. PubMed: 9383553 Thomas Lufkin, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |