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微信小章| 产品名称： | p63 |
|---|---|
| 商品货号： | TS139568 |
| Designations： | p63 |
| GenBank Number： | X06538 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.539999961853027 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.639999985694885 Tissue: breast adenocarcinoma MCF7 cell line Gene product: retinoic acid receptor, alpha RARA Alleles: A1, A2 |
| Insert Size (kb)： | 1.640 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Insert contains the following sites separated by (bp): KpnI- 380- PstI- 125- SacI- 495- PstI- 155- SmaI- 285- SmaI- 200- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; KpnI--5.0; EcoRI/KpnI--3.1, 1.8; SacI--5.0; PstI--3.7, 0.7, 0.5. Contains a 1296 nt open reading frame (starting at position 317) that encodes a 432 aa protein. RNA can be transcribed in vitro from vector T7 promoter. This appears to be a retinoic acid-inducible, trans-acting enhancer factor. Enzyme(s) not detecting polymorphism: ApaI, ApaLI, BamHI, BanI, BanII, BglII, BstNI, BxtXI, DraI, EcoO109, EcoRI, HindIII, MspI, NheI, PvuII, ScaI, StuI, TaqI, Tth111I, XmnI (in a minimum of 10 independent chromosomes). |
| References： | Mattei MG, et al. Mapping of the human retinoic acid receptor to the q21 band of chromosome 17. Hum. Genet. 80: 186-188, 1988. PubMed: 2844649 Zelent A, et al. Cloning of murine alpha and beta retinoic acid receptors and a novel receptor gamma predominantly expressed in skin. Nature 339: 714-717, 1989. PubMed: 2544807 Arveiler B, et al. A PstI RFLP for the human retinoic acid receptor in 17q21. Nucleic Acids Res. 16: 6252, 1988. PubMed: 2899875 Petkovich M, et al. A human retinoic acid receptor which belongs to the family of nuclear receptors. Nature 330: 444-450, 1987. PubMed: 2825025 Pierre Chambon, personal communication |