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| 产品名称： | p425 GAL1 |
|---|---|
| 商品货号： | TS139585 |
| Designations： | p425 GAL1 |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 7.5399999618530270 DESCRIPTION OF VECTOR: Intact vector size: 7.540 Type of vector: plasmid Cloning sites: SpeI BamHI SmaI PstI HindIII SalI XhoI Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI Construction: pRS425, GAL1 promoter Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): LEU2, ->, 200-2446 terminator: CYC1, ->, 3128-3388 MCS: XhoI...SpeI, ->, 3388-3445 promoter for expression: GAL1, marker(s): ampR, replicon: 2 micron, ->, 6093-7433 Vector: p425 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS425, GAL1 promoter Marker(s):LEU2,ampR Construct size (kb): 7.539999961853027 Features: marker(s): LEU2 marker(s): ampR promoter for expression: GAL1 replicon: 2 micron MCS: XhoI...SpeI terminator: CYC1 |
| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.9 (doublet); EcoRI--5.1, 2.4; KpnI--5.8, 1.7. It appears that the SacI site at the start of the GAL1 promoter has been lost. Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |