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| 产品名称： | pPH3 |
|---|---|
| 商品货号： | TS139621 |
| Designations： | pPH3 |
| Depositors： | T Su |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.5000000000000000 Vector: pPH3 (plasmid) Promoters: Promoter ugp Construction: ugp, pTER5, pUC19 Marker(s):ampR Construct size (kb): 2.5 Features: marker(s): ampR promoter: ugp replicon: pMB1 terminator: rpoCt, tL1 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--2.6; EcoRI--2.6; HindIII/EcoRI--2.6; BglII--1.8, 0.80. An expression vector useful in possibly all E. coli strains containing a functional pho regulatory circuit. The insert must supply the ribosome-binding site. Expression is repressed in the presence of excess inorganic phosphate (>1%) and high concentrations of glucose (>1%). Maximal induction was observed at limiting inorganic phosphate (<0.1 mM) and normal glucose (0.2-0.4%). The order of the major features in this plasmid is: L1 terminator - ugp promoter - EcoRI - MCS - HindIII - rpoC terminator - ori - bla. The nucleotide sequence of the transcription start point before the MCS is GCGAGCATAA AACGCGT. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Su TZ, et al. A novel phosphate-regulated expression vector in Escherichia coli. Gene 90: 129-133, 1990. PubMed: 2379833 |
| Shipped： | freeze-dried |