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微信小章| 产品名称： | Y-190 Y190 |
|---|---|
| 商品货号： | TS139629 |
| Designations： | Y-190 Y190 |
| Species： | Homo sapiens, human |
| Applications： | This probe is useful for detection of Y-derived marker chromosomes and mosaicism. |
| Vector： | Construct size (kb): 7.900000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 3.5 Tissue: 49,XXXXY cell line Gene product: DNA Segment, repetitive DYZ5 |
| Insert Size (kb)： | 3.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 3.5; EcoRI--6.1, 1.8; AvaI--4.7, 3.2; BamHI--8.0; PvuII--8.0. The sequences detected by Y-190 are present in numerous XX males. This probe hybridizes to Y-specific HindIII fragments of about 4 and 5 kb (strong) and 9 kb (weak) in the orangutan and 4 kb and 9 kb (weak) in the rhesus monkey. This probe detects 2 blocks of repeats on Yp. The hypervariable major block is composed of tandem 20.3 kb repeats with alleles ranging from about 540 to 800 kb (XbaI). The minor block is not variable and is about 60 kb. Detects a Y-specific 3.5 kb HindIII fragment that has a copy number of about 100 in normal human males. Also detects less repeated HindIII fragments ranging from 1.5 to 5.7 kb. This probe is useful for detection of Y-derived marker chromosomes and mosaicism. |
| References： | Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818 Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484 Muller U, et al. Y-190, a DNA probe for the sensitive detection of Y-derived marker chromosomes and mosaicism. Hum. Genet. 75: 109-113, 1987. PubMed: 3469135 Tyler-Smith C, et al. Structure of a hypervariable tandemly repeated DNA sequence on the short arm of the human Y chromosome. J. Mol. Biol. 203: 837-848, 1988. PubMed: 3210241 |