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| 产品名称： | pAL19 |
|---|---|
| 商品货号： | TS139630 |
| Designations： | pAL19 |
| Depositors： | H Okayama |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.0999999046325680 Vector: pAL19 (plasmid) Construction: pFL20, pDB262 Marker(s):LEU2,tetR Construct size (kb): 6.099999904632568 Features: insert detection: lacZ marker(s): LEU2 marker(s): tetR replicon: ars1 replicon: pMB1 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.2; HindIII--6.2; PstI--6.2. Plasmid linearized with PstI can be used to introduce cDNA libraries constructed in pcD or pcD2 vectors into Schizosaccharomyces pombe, resulting in the formation, by homologous recombination, of concatamers maintained as episomal DNA. Shares pBR322 sequences from the PvuII site to the ScaI site with pcD. Contains the ars sequence from Schizosaccharomyces pombe and the LEU2 gene from Saccharomyces cerevisiae. Insertions at the HindIII, BamHI, and SalI sites inactivate tetracycline resistance. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Okazaki K, et al. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res. 18: 6485-6489, 1990. PubMed: 2251111 |
| Related Products： | component of:ATCC 87686 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |