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| 产品名称： | pG28B5.0 |
|---|---|
| 商品货号： | TS139659 |
| Designations： | pG28B5.0 |
| GenBank Number： | U34070 |
| Species： | Homo sapiens, human |
| Depositors： | KG Xanthopoulos |
| Applications： | contains sequence CCAAT/enhancer binding protein (C/EBP), alpha |
| Vector： | Construct size (kb): 8.199999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-3Zf(+) Intact vector size: 3.199 Type of vector: phagemid Vector end: BamHI Vector end: BamHI Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII-SP6 Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CEBPA Gene name: CCAAT/enhancer binding protein (C/EBP), alpha Contains complete coding sequence?: Y Chromosome: 19; Localization: 19 q13.1 Tissue: umbilical vein endothelial cells Type of DNA: genomic Insert end: BamHI Insert end: BamHI Insert size (kb): 5.0 Cross references: DNA Seq. Acc.: U34070 Nucleotides ~700-4000 of the insert correspond to nucleotides 1-3318 of U34070. Insert lengths(kb): 5.0 Tissue: umbilical vein endothelial cells Gene product: CCAAT/enhancer binding protein (C/EBP), alpha CEBPA Target Gene: CCAAT/enhancer binding protein (C/EBP), alpha |
| Insert Size (kb)： | 5.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 3.2; HindIII--6.2, 1.5, 0.46; EcoRI--5.6, 2.5. Insert contains the following restriction sites (approximate kb from the 5 end): BglII--3.0; EcoRI--2.5; EcoRV--3.2; HindIII--3.1, 4.6; NcoI--1.3, 2.3; NotI--1.65, 1.7; PstI--0.7, 1.3, 1.85, 1.9, 4.4; SacI--2.3, 3.9; XbaI--3.5; XhoI--0.2, 2.35. The probe used for Northern blotting was the 0.7 kb EcoRI/HindIII fragment from the 3 untranslated region of the insert. |
| References： | Antonson P, Xanthopoulos KG. Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha). Biochem. Biophys. Res. Commun. 215: 106-113, 1995. PubMed: 7575576 |