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微信小章| 产品名称： | pYNH15 |
|---|---|
| 商品货号： | TS139713 |
| Designations： | pYNH15 |
| Species： | Homo sapiens, human |
| Applications： | Also detects a 2-allele RsaI RFLP (3.0, 1.7 kb) not tested in pedigree studies. |
| Vector： | Construct size (kb): 6.0 |
| Insert： | DNA: genomic Insert lengths(kb): 3.200000047683716 Gene product: DNA Segment, single copy D12S17 Alleles: A1, A2, A3 |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 2.2, 1.0. An EcoRI/HindIII double digest shows insert fragment sizes of 2.2 and 1.0 kb. The MspI RFLP shows significant linkage to autosomal recessive vitamin D dependency type I (thetam = 0.018, Z = 3.2). D12S17 and D12S25 detect the same polymorphism and therefore represent the same locus. Codominant segregation of the MspI polymorphism has been demonstrated in 42 3-generation families. Also detects a 2-allele RsaI RFLP (3.0, 1.7 kb) not tested in pedigree studies. Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, PstI, PvuII, TaqI. |
| References： | Labuda M, et al. Mapping autosomal recessive vitamin D deficiency type I to chromosome 12q14 by linkage analysis. Am. J. Hum. Genet. 47: 28-36, 1990. PubMed: 1971995 Hughes AE. D12S17 and D12S25 identify the same MspI RFLP. Nucleic Acids Res. 19: 1724, 1991. PubMed: 1709282 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pYNH15 on chromosome 12q D12S17. Nucleic Acids Res. 16: 779, 1988. PubMed: 2893347 Yusuke Nakamura, personal communication |