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| 产品名称： | K4-1.1 |
|---|---|
| 商品货号： | TS139714 |
| Designations： | K4-1.1 |
| Species： | Mus musculus, mouse |
| Depositors： | F Takei, KJ Horley |
| Vector： | Construct size (kb): 5.21999979019165 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.519999980926514 Tissue: myeloma NS-1 cell line Gene product: intercellular adhesion molecule 1 Ly47 |
| Insert Size (kb)： | 2.520 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains the following sites separated by (bp): EcoRI- 77- BstEII- 157- XhoI- 219- SacII- 539- PvuII- 147- HindIII- 547- PvuII- 640- PvuII- 145- Asp700I- 54- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--2.75, 2.6; BamHI--5.45; PstI--4.5, 0.52, 0.42; HindIII3.9, 1.55; XbaI--5.45. Contains a point mutation at position 183 (T to C) resulting in a Cys to Arg substitution. The mutant protein does not bind lymphocyte function antigen (LFA-1). Clones K4-1.1 (TS139714) and K3-1.1 (ATCC 63044) differ in the 5 end of the insert. Nt 638 of the K3-1.1 insert equals nt 132 of the K4-1.1 insert. The insert has an open reading frame of 1611 nt and 29 nt of 5 untranslated sequence. It codes for a functional transmembrane protein. The overall protein structure is quite similar to human ICAM1 and there is 50% homology at the amino acid level. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Horley KJ, et al. Molecular cloning of murine intercellular adhesion molecule (ICAM-1). EMBO J. 8: 2889-2896, 1989. PubMed: 2573511 Kathleen J Horley, personal communication Fumio Takei, personal communication |