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微信小章| 产品名称： | pMCT112 |
|---|---|
| 商品货号： | TS139798 |
| Designations： | pMCT112 |
| Species： | Homo sapiens, human |
| Applications： | A 1.9 kb SphI fragment of this clone has been used which is devoid of repetitive sequences. The AccI RFLP is observed under normal wash and hybridization stringencies. |
| Vector： | Construct size (kb): 5.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 3.599999904632568 Gene product: DNA Segment, single copy D9S15 Alleles: A1, A2, B1, B2 |
| Insert Size (kb)： | 3.600 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/EcoRI--2.8, 2.0, 0.96, + smaller bands; HindIII--2.8, 2.0, 0.96; EcoRI--6.0, 0.22. Codominant segregation demonstrated in 58 3-generation families for the MspI polymorphism. pMCT112 shows linkage to the Friedreich ataxia locus at a combined total LOD score of 25.09 at a recombination fraction of theta = 0. A single copy fragment of this clone detects the same 450 kb NotI fragment detected by D9S5. Detects genomic restriction fragments of the following sizes (complete digest, kb): NotI--450; EagI--100; BssHII--100; SacII--100. A 1.9 kb SphI fragment of this clone has been used which is devoid of repetitive sequences. The AccI RFLP is observed under normal wash and hybridization stringencies. Pre-association with excess human DNA is required. Enzyme(s) not detecting polymorphism: BglII, PstI, PvuII, RsaI, TaqI. Enzyme(s) not detecting polymorphism: BamHI, BclI, DraI, EcoRI, HincII, HindIII, HinfI, HpaI, KpnI, SalI, SstI, StuI, XbaI. |
| References： | Chamberlain S, et al. Genetic homogeneity at the Friedreich ataxia locus on chromosome 9. Am. J. Hum. Genet. 44: 518-521, 1989. PubMed: 2929596 Wallis J, Nakamura Y. A new AccI polymorphism for pMCT112 D9S15. Nucleic Acids Res. 17: 4904, 1989. PubMed: 2568612 Pandolfo M, et al. Friedreich ataxia in Italian families: genetic homogeneity and linkage disequilibrium with the marker loci D9S5 and D9S15. Am. J. Hum. Genet. 47: 228-235, 1990. PubMed: 2378348 Hanauer A, et al. The Friedreich ataxia gene is assigned to chromosoem 9q13-q21 by mapping of tightly linked markers and shows linkage disequilibrium with D9S15. Am. J. Hum. Genet. 46: 133-137, 1990. PubMed: 2294745 Fujita R, et al. Physical mapping of two loci (D9S5 and D9S15) tightly linked to Friedreich ataxia locus (FRDA) and identification of nearby CpG islands by pulse-field electrophoresis. Genomics 10: 915-920, 1991. PubMed: 1916823 Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence pMCT112 on chromosome 9q (D9S15). Nucleic Acids Res. 15: 10614, 1987. PubMed: 2892184 |