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| 产品名称： | pTacNeo3.2 CMCC 2302 |
|---|---|
| 商品货号： | TS139858 |
| Designations： | pTacNeo3.2 CMCC 2302 |
| Depositors： | JC Hunter-Cevera |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.4400000572204590 Vector: pTacNeo3.2 (plasmid) Promoters: Promoter tac Construction: pBR322, TAC promoter, lacZ, modified APH(3)-I Marker(s):ampR Construct size (kb): 5.440000057220459 Features: marker(s): ampR promoter: tac replicon: pMB1 |
| Applications： | expression vector shuttle vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.5; XhoI--5.5; PstI--3.2, 2.5. This is an expression vector capable of producing a beta-galactosidase: insert: APH(3)-I fusion protein. Sequences from the TAA stop codon of APH(3)-I to the immediate downstream PvuII site encode a portion of Tn601. Sequences from the PvuII site to the PstI site are derived from Tn5. The start codon of the modified APH(3)-I is followed by the eleventh codon (serine) of APH(3)-I. Codons 2-10 have been deleted. The upstream translation of LacZ is out of frame with APH(3)-I. The vector contains pBR322 (EcoRI-SphI), a TAC promoter, the first 8 amino-terminal codons of beta-galactosidase, and a gene encoding a modified aminoglycoside phosphotransferase (3)-I (APH(3)-I). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Jennie C Hunter-Cevera, personal communication |
| Shipped： | freeze-dried |