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| 产品名称： | CBRM1/23 |
|---|---|
| 商品货号： | TS139871 |
| Organism： | Mus musculus, mouse |
| Tissue： | spleen |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | BALB/c |
| Applications： | Pubmed: 7679388 The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580). Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. Pubmed: 7679388 The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact. Pubmed: 9614126 |
| Genes Expressed： | immunoglobulin; monoclonal antibody against
C-terminal epitope of human Mac-1 |
| Cellular Products： | immunoglobulin; monoclonal antibody against
C-terminal epitope of human Mac-1
|
| Comments： | Mice (BALB/c) were immunized with purified Mac1 (CD11b/CD18). Spleen cells were fused with P3X63Ag8.653 (ATCC CRL-1580). Monoclonal antibodies from selected hybridomas recognized the Mac-1 alpha subunits on the surface of neutrophils. Pubmed: 7679388 The monoclonal antibodies produced by these hybridomas will be new insights into how different domains in the integrin alpha and beta subunits may interact. Pubmed: 9614126 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(4) to 1 X 10(5) viable cells/ ml. Maintain cultures at a cell concentration between 5 X 10(5) and 1 X 10(6) cells/ml. |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | IgG2a; Kappa light chain |
| Name of Depositor： | TA Springer |
| Year of Origin： | November 27, 1991 |
| References： | Lu C, et al. The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. Dependence on beta subunit association and prediction of domains.. J. Biol. Chem. 273: 15138-15147, 1998. PubMed: 9614126 Diamond MS, et al. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands. J. Cell Biol. 120: 1031-1043, 1993. PubMed: 7679388 |