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| 产品名称： | pND201 |
|---|---|
| 商品货号： | TS139890 |
| Designations： | pND201 |
| Depositors： | NE Dixon |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.0130000114440920 Vector: pND201 (plasmid) Promoters: Promoter lambda PL Construction: pCE30 (ATCC 37830) Marker(s):ampR Construct size (kb): 4.013000011444092 Features: marker(s): ampR promoter: lambda PL, lambda PR replicon: pMB1 repressor gene: cI857 |
| Applications： | expression vector vector containing primer sites useful for sequencing vector for other uses |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.0; BamHI/EcoRI--4.0; BglI--2.9, 1.15. Potential inserts should be trimmed with Bal31 exonuclease to remove 5 sequences to within 10 bp of the start codon, and blunt-end ligated into the HpaI site downstream of a strong ribosome-binding site. Because cI857 is expressed by the plasmid itself, from its natural promoter PM, the vector may be used in virtually any E. coli strain. The sequence surrounding the cloning sites has been used to design primers for direct sequencing, including the M13 universal primer. Expression vector containing a strong ribosome-binding site and primer sites useful for sequencing. Encodes cI857. Constructed by inserting an oligonucleotide containing a HpaI site and a ribosome-binding site between the BamHI and SmaI sites of pCE30. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Elvin CM, et al. Modified bacteriophage lambda promoter vectors for overproduction of proteins in Escherichia coli. Gene 87: 123-126, 1990. PubMed: 2139621 |
| Shipped： | freeze-dried |