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| 产品名称： | pTUM1.4 |
|---|---|
| 商品货号： | TS139922 |
| Designations： | pTUM1.4 |
| Species： | Turnip mosaic virus |
| Depositors： | J Laliberte |
| Vector： | Construct size (kb): 4.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.447000026702881 Gene product: capsid, 33 kDa |
| Insert Size (kb)： | 1.447 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 0.9, 0.6; PstI--4.4; BamHI/HindIII--2.95, 1.45; XhoI--4.4; XbaI--3.0, 1.4. The insert contains the following restriction sites, in kb from the 5 end: ClaI--0.156; EcoRI--0.906. Isolated from naturally infected Brassica napus ssp. napobrassica (rutabaga) in the region of LAssomption, Quebec, Canada. Contains the complete capsid protein gene, part of the NIb gene, and part of the 3 end. The 5 end of the insert begins 204 bp 5 to the published sequence and end at nt -506 of the published sequence. Transcription from the T7 promoter gives the plus strand. |
| Classification： | Potyvirus |
| References： | Tremblay MF, et al. Sequence of the 3 terminal region of turnip mosaic virus RNA and the capsid protein gene. J. Gen. Virol. 71: 2769-2772, 1990. PubMed: 2254757 Jean-Francois Laliberte, personal communication |