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微信小章| 产品名称： | Trypanosoma sp. |
|---|---|
| 商品货号： | TS139945 |
| Strain Designations： | Indonesian 56 not cloned |
| Application： | Vector borne research |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | monkey, Macaca nemestrina, Indonesia, 1967 |
| Product Format： | frozen |
| Type Strain： | no |
| Medium： | ATCC® Medium 449: Diphasic blood agar base medium |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Subcultivation： | Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Cryopreservation： | 1.xa0xa0 Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min. 2. xa0 Adjust concentration of cells to 2 x 107/ml in fresh medium. 3. xa0 xa0While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in Lockex92s solution.xa0 The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use. 4. xa0 Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/ml and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min. 5. xa0 Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6.xa0xa0 Place the ampules in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0 7.xa0xa0 Store in either the vapor or liquid phase of a nitrogen refrigerator. 8.xa0xa0 To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after thawed. 9.xa0xa0 Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh tube of ATCC medium 1011.xa0 10.xa0xa0xa0xa0xa0xa0xa0xa0xa0 Incubate vertically at 25°C with the cap screwed on tightly. 11.xa0xa0xa0xa0xa0xa0xa0xa0xa0 Maintain as described above.xa0 |
| Name of Depositor： | D Weinman |
| Year of Origin： | 1967 |
| References： | Weinman D, Wiratmadja NS. The first isolates of trypanosomes in Indonesia and in history from primates other than man. Trans. R. Soc. Trop. Med. Hyg. 63: 497-506, 1969. PubMed: 5821271 |