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| 产品名称： | Feline infectious peritonitis virus |
|---|---|
| 商品货号： | TS139978 |
| Classification： | Coronaviridae, Alphacoronavirus, Alphacoronavirus 1 |
| Agent： | Feline infectious peritonitis virus |
| Strain： | FIPV-1146, P80 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | Live attenuated virus derived from serial passage of plaque-purified FIPV 79-1146, a strain isolated by J. Evermann.xa0 Intranasal administration of passage 80 protects adult cats from challenge by virulent virus.xa0 Cross reacts with canine coronavirus, TGE virus of swine and human coronavirus 229E. This item has been treated with Mycoplasma Removal Agent (MRA) to removexa0Mycoplasmaxa0at the ATCC. The previous item number was ATCC®xa0VR-2201™. |
| Effect on Host： | CPE, cell rounding and detachment |
| Recommended Host： | A-72 cells (ATCC® CRL-1542™) |
| Growth Conditions： | Temperature: 34°C Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer one time with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 34°C in air atmosphere, humidified, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 1-3 days at 34°C in air atmosphere, humidified, until CPE is progressed through 80% of the monolayer. At harvest, perform freeze-thaws to facilitate viral release. |
| Name of Depositor： | FW Scott |
| Source： | FIPV-WSU-1146 isolated from necropsy of a feline neonate with pneumonia and pleuritis |
| References： | Baldwin CA, Scott FW. Feline infectious peritonitis vaccine and method of preparation. US Patent 5,043,281 dated Aug 27 1991 McKeirnan AJ, et al. Isolation of Feline Coronaviruses from Two Cats with Diverse Disease Manifestations. Feline Pract. 11: 16-20, 1981. Pederson et al., Adv. Exp. Med. Biol. 173: 365-380, 1984 and US Pat. 4,195,130. |