微信小陈
微信小章| 产品名称： | pHPT31 |
|---|---|
| 商品货号： | TS140016 |
| Designations： | pHPT31 |
| Species： | Homo sapiens, human |
| Insert： | DNA: cDNA Insert lengths(kb): 0.949999988079071 Tissue: central nervous system Gene product: hypoxanthine phosphoribosyltransferase HPRT Alleles: A1, A2, A3 |
| Insert Size (kb)： | 0.950 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.5, 1.0; SalI--2.25, 0.95; PstI--2.5, 0.93; EcoRI--2.4, 0.99. The clone was constructed by excising the PstI fragment encoding the full-length cDNA from pHPT30 and inserting the fragment into pUC7. The insert includes about 100 bp 5 untranslated and some 3 untranslated sequence, but not the AATAAA sequence. The insert detects the same BamHI polymorphism detected by the probe, pDSK1. Enzyme(s) not detecting polymorphism: EcoRI, HindIII, MspI, KpnI, TaqI. |
| References： | Jolly DJ, et al. Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 80: 477-481, 1983. PubMed: 6300847 Brennand J, et al. Expression of human and Chinese hamster hypoxanthine-guanine phosphoribosyltransferase cDNA recombinants in cultured Lesch-Nyhan and Chinese hamster fibroblasts. J. Biol. Chem. 258: 9593-9596, 1983. PubMed: 6688416 Melton DW, et al. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene. Proc. Natl. Acad. Sci. USA 81: 2147-2151, 1984. PubMed: 6326107 C. Thomas Caskey, personal communication |