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| 产品名称： | pNKY150 Tn10 URA Blaster |
|---|---|
| 商品货号： | TS140076 |
| Designations： | pNKY150 Tn10 URA Blaster |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | N Kleckner |
| Applications： | A plasmid containing an excisable URA3 cartridge for gene disruption that permits repeated use of URA3 selection in constructing multiply disrupted yeast strains. |
| Vector： | Construct size (kb): 8.5 |
| Insert： | DNA: genomic Insert lengths(kb): 4.599999904632568 Gene product: uridine monophosphate synthetase( UMP synthase, orotate phosphoribosyltransferase, orotidine 5-phosphate decarboxylase, orotate phosphoribosyltransferase 1) URA3 |
| Insert Size (kb)： | 4.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/BglII--5.0, 4.2; BamHI--9.2; EcoRI--9.2; PstI--6.6, 2.6; XbaI--uncut. Used by inserting the excised cassette into a cloned target gene, isolating a restriction fragment containing the cassette with flanking target gene sequences, transforming Ura3- strains, and selecting for stable integrants. Loss of the cassette (and one of the flanking IS10 repeats) by recombination can be selected by 5-fluoro-orotic acid resistance. A plasmid containing an excisable URA3 cartridge for gene disruption that permits repeated use of URA3 selection in constructing multiply disrupted yeast strains. The BglII/BamHI cassette contains URA3 flanked by 1.5 kb direct repeats from insertion sequence IS10. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Alani E, et al. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545, 1987. PubMed: 3305158 Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 |