产品名称: | pmPit1 |
---|---|
商品货号: | TS140097 |
Designations: | pmPit1 |
Species: | Mus musculus, mouse |
Depositors: | SA Camper |
Vector: | Construct size (kb): 3.630000114440918 |
Insert: | DNA: cDNA Insert lengths(kb): 0.671999990940094 Tissue: pituitary Gene product: pituitary specific transcription factor-1 Pit1 |
Insert Size (kb): | 0.672 |
Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: freeze-dried |
Comments: | The insert contains RsaI sites 479 nt and 642 nt from the BamHI cloning site. Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--3.0, 0.67; EcoRI--3.6; HindIII--3.6; BamHI--3.6; PstI--uncut. Mapped to chromosome 16 by linkage with dw (2.7 cM at 95% confidence level). The orientation in the vector is such that aa 47 (the 5 end) is at the BamHI site and aa 269 (the 3 end) is at the HindIII site. Cloned by PCR amplification from cDNA using primers with HindIII and BamHI linkers. The product corresponds to aa 47 to aa 269. |
Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
References: | Camper SA, et al. The Pit-1 transcription factor gene is a candidate for the murine snell dwarf mutation. Genomics 8: 586-590, 1990. PubMed: 1981057 Sally A Camper, personal communication |