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| 产品名称： | NU-DUL-1 |
|---|---|
| 商品货号： | TS140166 |
| Organism： | Homo sapiens, human |
| Tissue： | peritonial effusion; derived from metastatic site: malignant cerebrospinal fluid |
| Cell Type： | lymphocyte |
| Product Format： | frozen |
| Morphology： | early B-cell |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | undifferentiated lymphoma, non-Burkitts type |
| Age： | 43 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | The Nu-DUL-1 cell line is a valuable resource for studying the biological behavior of undifferentiated lymphoma cells. The Nu-DUL-1 cell line will aid molecular and cellular studies designed to identify the biological significance of genomic rearrangements. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | 44, X (hypodiploid), +der(4)t(4;?)(q31;?). -6, -17, -18, -21, +2mar,der(1)t(1;?)(q32;?), del(4)(q25q31), del(5)(p12), del(6)(q21), del(8)(q24), der(11)t(11;?)(q21;?), der(12)t(12;?)(p13;?), der(14)t(14;?)(q13;), ter rea(19;21)(q13;q22) |
| Images： |  |
| Clinical Data： | 43 years Caucasian Male |
| Antigen Expression： | LN-1+ LN-2+ LN-3+(anti-HLA-Dr) BA-1+ (CD24) CALLA (J5)(common ALL antigen; CD10)+ BA-2 - (CD9) OKM1 - (myeloid marker) Leu-7 - (natural killer T cell marker) OKT-3 -, OKT-4 -, OKT-6 -, OKT-8 -, OKT-11 - (T cell markers) BM-1 -, BM-2 -, BM-3 - ,BM-4 -, BM-5 - (myeloid markers) EBNA - (Epstein-Barr Nuclear Antigen) |
| Genes Expressed： | sIg - |
| Comments： | The depositor states that this cell line is negative for the Epstein-Barr Virus (EBV). ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR. The NU-DUL-1 cell line represents a very unusual undifferentiated lymphoma. The NU-DUL-1 cell line has a chromosome 14 translocation, but not from chromosome 11 or 18 and may represent a new variant. This cell line is hypodiploid with the presumed loss of a Y chromosome. NU-DUL-1 cells express markers of early B-cell differentiation. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium. An inoculum of 2.0 x 105to 3.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 105 and 1.0 x 106 cells/mL. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:5 is recommended. Medium renewal: every 2 to 3 days |
| Cryopreservation： | Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 12, 14 |
| Name of Depositor： | A Epstein |
| Year of Origin： | 1985 |
| References： | Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794 |