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微信小章| 产品名称： | Monkey cytomegalovirus |
|---|---|
| 商品货号： | TS140167 |
| Classification： | Herpesviridae, Cytomegalovirus |
| Agent： | Monkey cytomegalovirus |
| Strain： | 68-1 |
| Common Name： | African green monkey cytomegalovirus |
| Applications： | Produces enlarged, distorted nuclei with bizarre inclusions infected human and monkey fibroblast TC. Has morphology of the herpes group (by EM). |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Urine of rhesus monkey, 1968 |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Comments： | Sensitive to heat (exposure to 56C for 10 min. completely inactivated 10(4) TCID50 of Rhesus CMV). Produces enlarged, distorted nuclei with bizarre inclusions infected human and monkey fibroblast TC. Has morphology of the herpes group (by EM). |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects (refractile rounding and sloughing) CPE, refractile rounding and sloughing |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
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| Recommended Host： | Recommended Host:xa0 MRC-5 (ATCC CCL-171) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 35.0°C Duration: 3 to 30 days; For best results, cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent) at inoculation. |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | DM Asher |
| Source： | Urine of rhesus monkey, 1968 |
| Year of Origin： | 1968 |
| References： | Kaur A, et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893 Asher DM. . Bacteriol. Proc. 269: 91, 1969. Asher DM, et al. Persistent shedding of cytomegalovirus in the urine of healthy Rhesus monkeys. Proc. Soc. Exp. Biol. Med. 145: 794-801, 1974. PubMed: 4131945 or colder almost any human or monkey diploid fibroblast tissue culture cells may be used or colder |