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微信小章| 产品名称： | pLC4-14 RG100MetJ |
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| 商品货号： | TS140277 |
| Designations： | pLC4-14 RG100MetJ |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | JR Uren |
| Applications： | This strain overproduces beta-cystathionase 100-fold over wild type levels. |
| Vector： | Construct size (kb): 0.0 |
| Insert： | DNA: genomic Gene product: cystathionase beta metC |
| Media： | ATCC Medium 1065 (see below) plus streptomycin (100.0 mcg/ml) plus thymidine (50.0 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
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| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the plasmid |
| Comments： | This strain overproduces beta-cystathionase 100-fold over wild type levels. This clone was isolated from the Clarke-Carbon Escherichia coli library, constructed of hybrid Col E1 plasmid - Escherichia coli DNA using poly(dA-dT) connectors. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Su CH, Greene RC. Regulation of methionine biosynthesis in Escherichia coli: Mapping of the metJ locus and properties of a metJ+/metJ- diploid. Proc. Natl. Acad. Sci. USA 68: 367-371, 1971. PubMed: 5277087 Dwivedi CM, et al. Cloning, purification, and characterization of beta-cystathionase from Escherichia coli. Biochemistry 21: 3064-3069, 1982. PubMed: 7049234 Clarke L, Carbon J. A colony bank containing synthetic Col E1 hybrid plasmids representative of the entire E. coli genome. Cell 9: 91-99, 1976. PubMed: 788919 Jack R Uren, personal communication |