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微信小章| 产品名称： | Human rhinovirus 31 |
|---|---|
| 商品货号： | TS140294 |
| Classification： | Picornaviridae, Rhinovirus, Human rhinovirus A |
| Agent： | Human rhinovirus 31 |
| Strain： | 140 F |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | This item has been treated with diethyl ether to remove Mycoplasma orale at the ATCC.xa0 The previous item number was VR-506™ |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | HeLa (ATCC® CCL-2™) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.005 - 0.01 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, while rocking. End adsorption by adding virus growth medium. Incubation: 1-7 days at 33°C in a humidified 5% CO2 atmosphere while rocking, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | VV Hamparian and RM Conant |
| Source： | Human throat washings |
| References： | Conant RM, and Hamparian VV. Rhinoviruses: Basis for a Numbering System. I. HeLa cells for propagation and serologic procedures. J Immunol 100 (1): 107-113, 1968. PubMed: 4295059 Conant RM, and Hamparian VV. Rhinoviruses: Basis for a Numbering System. II. Serologic characterization of prototype strains. J Immunol 100 (1): 114-119, 1968. PubMed: 4295060 Connelly AP Jr., Hamre D. Virologic studies on acture respiratory disease in young adults. II. Characteristics and serolotic studies of three new Rhinoviruses. J. Lab. Clin. Med. 63: 30-43, 1964. PubMed: 14102901 |