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| 产品名称： | p2UGCAT |
|---|---|
| 商品货号： | TS140335 |
| Designations： | p2UGCAT |
| Depositors： | KR Yamamoto |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.5999999046325680 Vector: p2UGCAT (plasmid) Promoters: Promoter CYC1 Construction: p2UG, CAT Marker(s):URA3,ampR Construct size (kb): 7.599999904632568 Features: marker(s): ampR, URA3 promoter: CYC1 replicon: pMB1, 2 micron enhancer: GRE |
| Applications： | YE-type (episomal) shuttle vector expression vector reporter construct shuttle vector vector with sequences for protein export |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--7.6, EcoRI--2.2 (doublet), 1.7, 1.4; KpnI--7.6. The URA3 marker is from S. cerevisiae but complements the ura4 allele from Sc. pombe. Expression of chloramphenicol acetyltransferase is hormone-inducible in both S. cerevisiae and Sc. pombe hosts, in the presence of a glucocorticoid receptor such as that encoded by pG-N795 (ATCC 37813) or pART1/N795 (ATCC 37814). Contains the CYC1 TATA region from S. cerevisiae (nt -178 to +49) fused at nt -178 to 3 copies of a 26 bp glucocorticoid response element (GRE) from the rat tyrosine aminotransferase gene. A BglII/BamHI fragment with a chloramphenicol acetyltransferase coding region, its own AUG codon, and some SV40 sequences was cloned into the BamHI site of the polylinker. The other polylinker sites are 3 to this insert. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Picard D, et al. An inducible expression vector for both fission and budding yeast. Gene 86: 257-261, 1990. PubMed: 2108906 |
| Shipped： | freeze-dried |