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| 产品名称： | pMCR3 |
|---|---|
| 商品货号： | TS140340 |
| Designations： | pMCR3 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | The probe was pre-associated with total human DNA. Otherwise, RFLPs were observed under normal hybridization and wash stringencies |
| Vector： | Construct size (kb): 12.0 |
| Insert： | DNA: genomic Insert lengths(kb): 6.0 Gene product: neuroblastoma RAS viral (v-ras) oncogene homolog( v-ras neuroblastoma RAS viral oncogene homolog, proto-oncogene N-ras) NRAS Alleles: A1, A2 |
| Insert Size (kb)： | 6.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.65, 2.8, 2.8; HindIII--3.85, 3.85, 2.9, 0.86, 0.45; PstI--5.7, 3.1, 2.3, 1.6; BamHI--6.6, 3.0, 2.7, indicating two vectors + insert. Codominant segregation demonstrated in 55 3-generation families for the EcoRI RFLP. The probe was pre-associated with total human DNA. Otherwise, RFLPs were observed under normal hybridization and wash stringencies Enzyme(s) not detecting polymorphism: MspI, TaqI, RsaI, BglII, PstI, PvuII, BamHI, HindIII. |
| References： | Hall JM, et al. Oncogenes and human breast cancer. Am. J. Hum. Genet. 44: 577-584, 1989. PubMed: 2564734 Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294 Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence for NRAS pMCR3 on chromosome 1. Nucleic Acids Res. 15: 9623, 1987. PubMed: 2891121 |