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| 产品名称： | YIp358R |
|---|---|
| 商品货号： | TS140344 |
| Designations： | YIp358R |
| Depositors： | CJ Lusty |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0999999046325680 Vector: YIp358R (plasmid) Promoters: Promoter none Construction: YEp358R (ATCC 37739), YIp352 Marker(s):URA3,ampR Construct size (kb): 7.099999904632568 Features: insert detection: lacZ marker(s): ampR, URA3 promoter: none replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector promoter-cloning vector shuttle vector |
| Comments： | The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): HindIII-2; SphI-3; PstI-3; SalI-2; XbaI-2; BamHI-2; SmaI-3; KpnI-3; SacI-3; EcoRI-2. The SacI site is not unique. Cloning into the HindIII, SphI, PstI, SalI, or XbaI sites leads to a TAG stop codon within the downstream XbaI site of the multiple cloning region. Restriction digests of the clone give the following sizes (kb): EcoRI-7.1; SalI-7.1; PstI-7.1; HindIII-7.1. One of 3 promoter-cloning, YI type shuttle vectors (ATCC 37755 - 37757) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The sequence and reading frame of the multiple cloning sequence is: 5AA GCT TGC ATG CCT GCA GGT CGA CTC TAG AGG ATC CCC GGG TAC CGA GCT CGA ATT CCC AGC TTC GAT CCC3, from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915 |
| Shipped： | freeze-dried |