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| 产品名称： | pYNM3 |
|---|---|
| 商品货号： | TS140376 |
| Designations： | pYNM3 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | RFLPs observed under normal stringency; washes at 0.1X SSC, 0.1% SDS, 65 C. |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: genomic Insert lengths(kb): 2.799999952316284 Gene product: DNA Segment, single copy D8S17 |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): SalI--5.6, PstI--2.77, 1.5, 1.39, 1.0; HindIII--5.6; EcoRI--2.6 doublet; BamHI--4.8, 0.53. The PstI polymorphism demonstrates codominant segregation in more than 50 families. RFLPs observed under normal stringency; washes at 0.1X SSC, 0.1% SDS, 65 C. Enzyme(s) not detecting polymorphism: MspI, TaqI. |
| References： | Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872 Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pYNM3 on chromosome 8 (D8S38). Nucleic Acids Res. 15: 10080, 1987. PubMed: 2892174 Peter J OConnell, personal communication |