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| 产品名称： | pOM4 |
|---|---|
| 商品货号： | TS140430 |
| Designations： | pOM4 |
| Depositors： | I Oberbaumer |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.7130000591278080 Vector: pOM4 (plasmid) Promoters: Promoter lac Construction: pUC8, M13tg131 Marker(s):ampR Construct size (kb): 2.713000059127808 Features: insert detection: lacZ marker(s): ampR promoter: lac replicon: pMB1 |
| Applications： | expression vector vector containing primer sites useful for sequencing vector for constructing cDNA libraries vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7; ClaI--2.7. Escherichia coli transformed with this plasmid produce larger colonies when an insert is present in the polylinker than when there is no insert. Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation. pOM3 (ATCC 77222) and pOM4 (TS140430) differ in the orientation of the polylinker. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975 Oberbaumer I. Blue libraries are beautiful. Focus 14: 72, 1992. Ilse Oberbaumer, personal communication |
| Shipped： | freeze-dried |