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| 产品名称： | HIBBS11 |
|---|---|
| 商品货号： | TS140432 |
| Designations： | HIBBS11 |
| GenBank Number： | T09304 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.599999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: lafmid BA Intact vector size: 3.153 Type of vector: phagemid Vector end: HindIII Vector end: NotI Cloning sites: HindIII, BamHI, NotI, EcoRI Polylinker sites: HindIII, BamHI, NotI, EcoRI Other unique sites: ClaI, XmnI, ScaI, ApaLI Construction: pGEM Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, insert detection: lacZ, primer site: M13 universal, -> MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098 primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CHP* Gene name: chaperonin HumAP (Cct, TCP1-related) Contains complete coding sequence?: N Sequence homology: Homo sapiens t complex-related protein-1, related sequence 3 (matricin) Tissue: infant brain, 3 months old (from Bento Soares) Type of DNA: cDNA Insert 5 end: HindIII Insert 3 end: NotI Insert size (kb): 1.3 Cross references: DNA Seq. Acc.: L20509 Nucleotides 1-364 of the insert correspond to nucleotides 709-1072 of L20509 with 90% identity. DNA Seq. Acc.: T09304; EST: EST07197; Primer: M13 Reverse; THC id: THC123995 DNA Seq. Acc.: X74801 Nucleotides 1-364 of the insert correspond to nucleotides 626-989 of X74801 with 98% identity. Insert lengths(kb): 1.299999952316284 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: chaperonin HumAP (Cct, TCP1-related) CHP* |
| Insert Size (kb)： | 1.300 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/NotI--3.3, 1.3; EcoRI--3.6, 1.1. Based on the size of the insert and the alignment with GenBank X74801 (1901 nt, CDS 1-1635), this clone appears to contain part of the coding region. The cDNAs (>95%) are cloned directionally with the 5 end adjacent to the M13 reverse primer and HindIII. |
| References： | Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585 Mark D Adams, personal communication |