Construct size (kb): 5.699999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CNR1
Other gene symbols: CNR
Gene name: cannabinoid receptor 1 (brain)
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 q14-q15
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.75
Cross references: DNA Seq. Acc.: M77952; EST: EST01536; Primer: M13 Forward;
THC id: THC93435

Insert lengths(kb): 2.75

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: cannabinoid receptor 1 (brain) CNR

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 2.15, 0.6; BamHI--5.7; PstI--3.3, 0.95, 0.75, 0.7.

EST01536 matches Swiss-Prot accession CANR$HUMAN with 93.87% identity and 96.93% similarity over the sequence range: 385-92.