产品名称: | 2+6+11 |
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商品货号: | TS140541 |
Designations: | 2+6+11 |
GenBank Number: | Z22551 |
Species: | Homo sapiens, human |
Applications: | contains sequence kinectin |
Vector: | Construct size (kb): 7.300000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-7Zf(+) Intact vector size: 3.000 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII HindIII BamHI SacI BstXI NsiI Polylinker sites: ApaI AatII SphI XbaI XhoI EcoRI KpnI SmaI AsuII ClaII HindIII BamHI SacI BstXI NsiI Host range: Escherichia coli Features (with orientation and position when available): enhancer: none insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1, f1 terminator: none Cross references: |
Insert: | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: KIN* Gene name: kinectin Contains complete coding sequence?: Y Type of DNA: cDNA Insert end: Modification: EcoRI adaptor Insert end: Modification: EcoRI adaptor Insert size (kb): 4.309 Cross references: DNA Seq. Acc.: Z22551 Nucleotides 1-4309 of the insert correspond to nucleotides 1-4309 of Z22551. Insert lengths(kb): 4.309000015258789 Gene product: kinectin KIN* Target Gene: kinectin |
Insert Size (kb): | 4.309 |
Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information: | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
Comments: | Restriction digests of the clone give the following sizes (kb): BamHI--7.0, 1.0; EcoRI--4.7, 3.4; XhoI--4.4, 3.7. Insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.85; BglII--3.76; HindIII--0.20, 0.70, 1.67; KpnI--0.62; NcoI--0.07; PstI--1.40, 3.21; PvuII--0.54, 2.95; XbaI--3.42; XhoI--0.31. Recommendation for verification: EcoRI--4.3, 3.0; BamHI--6.4, 0.9; HindIII--5.6, 1.0, 0.5, 0.2; XbaI--6.4, 0.9; XhoI--4.0, 3.3. |
References: | Futterer A, et al. Molecular cloning and characterization of human kinectin. Mol. Biol. Cell 6: 161-170, 1995. PubMed: 7787243 |