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| 产品名称： | pHisGal |
|---|---|
| 商品货号： | TS140580 |
| Designations： | pHisGal |
| Depositors： | ML Schmitz |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.9119999408721920 Vector: pHisGal (phagemid) Promoters: Promoter for expression lac Construction: pQE9, Gal4 DNA-binding domain Marker(s):ampR Construct size (kb): 3.911999940872192 Features: marker(s): ampR operator: Gal4 DNA-binding domain (aa 1-147) promoter for expression: lac replicon: pMB1 MCS: SmaI...HindIII restriction site: BamHI coding sequence: promoterless cat gene epitope tag: hexahistidine sequence (6-His) |
| Applications： | expression vector vector permitting construction of fusion proteins |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.0; PvuI--4.0; XbaI--4.0. Plasmid contains the following restriction sites (approximate kb from the BamHI site): XbaI--1.51; PvuI--3.15. Expression vector for production of Gal4 fusion proteins, allowing protein purification by affinity chromatography through a nickel nitrilotriacetic acid (Ni-NTA) column. For expression of cloned inserts, the medium should contain 10 uM zinc chloride and expression can be induced using IPTG. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Schmitz ML, Baeuerle PA. A vector, pHisGal, allowing bacterial production of proteins fused to a hexahistidine-tagged Gal4 DNA-binding domain. BioTechniques 17: 714-718, 1994. PubMed: 7833034 |
| Shipped： | freeze-dried |