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| 产品名称： | pBTI-1 |
|---|---|
| 商品货号： | TS140597 |
| Designations： | pBTI-1 |
| Depositors： | DW Morris |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.0000000000000000 Vector: pBTI-1 (cosmid) Construction: MUA-10, YEp13 Marker(s):LEU2,ampR,tetR Construct size (kb): 11.0 Features: marker(s): ampR, tetR, LEU2 replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector shuttle vector vector for constructing cDNA libraries |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. A Saccharomyces cerevisiae/Escherichia coli cosmid shuttle vector. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Morris DW, et al. Plasmid vectors capable of transferring large DNA fragments to yeast. DNA 1: 27-36, 1981. PubMed: 6299664 |
| Shipped： | freeze-dried |