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| 产品名称： | PKCepsilon(E3) |
|---|---|
| 商品货号： | TS140670 |
| Designations： | PKCepsilon(E3) |
| GenBank Number： | X65293 |
| Species： | Homo sapiens, human |
| Depositors： | DJ Burns |
| Applications： | in another host, produces protein protein kinase C, epsilon |
| Vector： | Construct size (kb): 16.20000076293945 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBlueBac Intact vector size: 14.000 Type of vector: phagemid Vector end: NheI Vector end: NheI Cloning sites: NheI Polylinker sites: Host range: insect cells; Escherichia coli Features (with orientation and position when available): insert detection: lac marker(s): ampR promoter: polyhedrin replicon: pMB1, f1, SV40 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: PRKCE Gene name: protein kinase C, epsilon Contains complete coding sequence?: Y Tissue: brain Type of DNA: cDNA Insert end: NheI Insert end: NheI Insert size (kb): 2.2 Cross references: DNA Seq. Acc.: X65293 Insert lengths(kb): 2.200000047683716 Tissue: brain Gene product: protein kinase C, epsilon PRKCE Target Gene: protein kinase C, epsilon |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): NheI--14.0, 2.3; HindIII--9.5, 4.4, 1.1, 1.0, 0.4; PstI--14.0, 1.3, 0.8, 0.2; PvuII--3.5, 3.1, 2.5, 2.4, 1.6, 1.4, 1.25, 0.4; SmaI--14.0, 2.1. The insert contains the following restriction sites (approximate kb from the 5 end): HindIII--1.92; PstI--0.32, 1.54, 1.74; PvuII--1.16. Constructed from the 5NcoI/3EcoRI fragment (nt 14-2185 of the sequence record) using NheI adaptors. |
| References： | Basta P, et al. Sequence and expression of human protein kinase C-epsilon. Biochim. Biophys. Acta 1132: 154-160, 1992. PubMed: 1382605 David J Burns, personal communication |