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| 产品名称： | pHA68B12 68B12, pHSA68B12 |
|---|---|
| 商品货号： | TS140680 |
| Designations： | pHA68B12 68B12, pHSA68B12 |
| Species： | Homo sapiens, human |
| Depositors： | G Lucotte |
| Applications： | THe second variant has been validated in family studies. |
| Vector： | Construct size (kb): 5.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.0 Tissue: liver Gene product: albumin ALB Alleles: E1, C1, C2, D1, F1, F2, A1, D2, E2, A2 |
| Insert Size (kb)： | 1.0 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Insert contains internal PstI (1), TaqI (2), MboII (2), HhaI (1), HinfI (2), HindIII (1), XbaI (1), and HincII (1) sites. Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.9; EcoRI/NdeI--3.0, 2.3; EcoRI--5.3; HindIII--3.8, 1.5; BamHI--5.3. The insert is from the 3 half of the cDNA. No HaeIII is known in the cDNA of the albumin gene, so the variants must be intronic. In one variant, 2 of 42 or f=0.05 were missing the 2.7 and 1.6 kb bands. In another variant, bands 4, 2.7, 0.7, 0.65, 0.6, and 0.55 were missing (f= 0.21). THe second variant has been validated in family studies. When DNA is digested with HaeIII, the probe detects bands at 4, 3.4, 2.9, 2.7, 1.6, 0.95, 0.75, 0.72, 0.65, and 0.6 kb. |
| References： | de Souza SL, et al. Pathologie moleculaire des genes albumine et alpha-foetoproteine. Hum. Genet. 67: 48-51, 1984. PubMed: 6086494 Mariotti M, et al. Individual polymorphism of albumin gene studied by sequencing data. Protides Biol. Fluids Proc. Colloq. 33: 177-179, 1985. Lavareda de Souza S, et al. Polymorphisms of human albumin gene after restriction with HaeIII. Bio-sciences 5: 71-74, 1986. Gerard Lucotte, personal communication |