宁波泰斯拓生物

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浙江省宁波市镇海区庄市街道兴庄路9号创e慧谷42号楼B幢401室
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human c-cbl

货号 TS140730
中文名称 null
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产品名称: human c-cbl
商品货号: TS140730
Designations: human c-cbl
GenBank Number:

X57110

Species: Homo sapiens, human
Vector:
Construct size (kb): 5.599999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC
Intact vector size: 2.700
Type of vector: plasmid
Vector end: SmaI
Vector end: PstI
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CBL
Other gene symbols: CBL2
Genomic copy number: unique
Gene name: Cas-Br-M (murine) ecotropic retroviral transforming
sequence
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 q23.3-qter
Tissue: HUT-78 and CCRF-CEM cell lines
Type of DNA: cDNA
Insert 5 end: ApaI
Insert 3 end: PstI
Insert size (kb): 2.9
Cross references: DNA Seq. Acc.: X57110
Nucleotides 1-2886 of the insert correspond to
nucleotides 1-2886 of X57110.
Insert lengths(kb): 2.900000095367432
Tissue: HUT-78 and CCRF-CEM cell lines
Gene product: Cas-Br-M (murine) ecotropic retroviral transforming sequence(Cas NS-1 viral (v-cbl) oncogene homolog) CBL
Insert Size (kb): 2.900
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): BglI--5.6; EcoRI--3.2, 2.4; PstI--2.9, 2.7; SacI--3.0, 1.5, 1.3.
The insert contains the following restriction sites (approximate kb from the 5 end): SacI--0.17, 1.5; EcoRI--0.58; HindIII--0.72, 1.62, 2.78; BglII--1.77.
References:

Blake TJ, et al. The sequences of the human and mouse c-cbl proto-oncogenes show v-cbl was generated by a large truncation encompassing a proline-rich domain and a leucine zipper-like motif. Oncogene 6: 653-657, 1991. PubMed: 2030914

Wallace Y Langdon, personal communication